Best Time to Inject Testosterone – Is Morning or Night Better?

  • By: Dave Moffat
  • Date: December 8, 2023
Best Time to Inject Testosterone

A lot of people ask if the time of day matters when you take testosterone shots. Does it make a big difference?

Research shows that it doesn’t really matter if you take the shot in the morning or at night.

What is really important is sticking to a regular schedule for your shots to make sure you’re getting the right amount each time.

For example, taking a shot every Monday morning and Thursday evening could work well for most people.

It’s not so much about when you take the shot, but making sure you take it the right way and on a regular schedule.

Remember, once you take the shot, the testosterone will stay in your body for a certain amount of time, no matter when you took it.

Testosterone Levels and Timing Your Injections

Some people think that the time of day can change how well testosterone shots work. But guess what? That’s not true. Scientists have found out that it doesn’t really matter what time you get these shots. They work the same way whether it’s morning or night.

The important thing is that doctors give these shots based on how much testosterone you have in your body right now, not what time it is.

Every person is different, so doctors should make a special plan for each person to make sure they get the best results from their shots.

Ester Forms of Testosterone and Half-Life 

If you’re starting on testosterone replacement therapy (TRT), one of the first things you need to know is how long testosterone stays in your body. This is called its half-life.

There are different types of testosterone you can use. Most people pick cypionate because it works slowly and you inject it into your body. Cypionate lasts for about 2 weeks in your bloodstream and has an 8-day half-life. So, you’ll likely need to take shots once or twice a week. Some people might choose to take smaller doses more often throughout the week.

Another type, testosterone propionate, doesn’t stay in your body as long – only 2 days. That means you’d need to take shots every 3rd or 4th day to keep a steady level in your body.

Enanthate is another type of testosterone that you can split into multiple doses, like 2 x 60 mg a week. Doing this can give you a steady supply of testosterone without overloading your body.

When using propionate or enanthate, split your weekly dose into multiple shots. This helps maintain steady hormone levels throughout the week.

Taking shots more often, like twice a week, might help lower the chance of having too much estrogen.

Remember, your testosterone levels will be highest 24–72 hours after you take a shot and will go back to normal 10–14 days after the shot.

If you need smaller doses each week or if you have a larger body, you might benefit from taking shots three times a week.

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But be careful not to take shots too often because this could increase the chance of side effects. Always decide your next dose based on the last one you took.

Considerations When Deciding When to Inject Testosterone 

For people who take medicine by injecting it, when they do it can really change how they feel. If they inject at night, they might have trouble sleeping. This could be because they feel anxious or excited from the injection.

On the other hand, some people might like to inject in the early morning. This could make them feel different.

When you inject, remember the energy you feel is from excitement, not testosterone.

Some people might find that injecting in the morning messes up their work and sleep schedules. Others might like injecting at night because it makes the soreness at the injection site feel better since they move less.

Injecting under the skin is usually best for getting a steady dose. But it’s important to do it right. You should hold the needle at a 45-degree angle and avoid pinching your skin.

The best time for a testosterone injection varies based on individuals. Morning, evening, or night – it’s personal preference. Everyone’s body reacts differently, so find what works for you.

It’s always a good idea to talk to a doctor when deciding how and when to take your medicine.

By working with a doctor, people can figure out the best time and amount for their testosterone injections to get the best results.

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The Best Time to Inject Testosterone – Factors to Consider

Are you considering testosterone injections to help increase your testosterone levels? If so, deciding on the best time to inject can help maximize the effectiveness of treatment. When deciding when to inject, consider testosterone levels, injection frequency, exercise timing, and time of day.

Body’s natural testosterone levels

When deciding on the best time to inject testosterone, it’s essential to focus on naturally occurring testosterone peaks and valleys in the body. Testosterone levels naturally rise in response to sexual activity, exercise, and waking up in the morning. As such, it is ideal to inject testosterone in the morning, as this is when levels are naturally higher.

Injection frequency

When deciding on the best time to inject testosterone, consider your injection frequency. When injecting weekly, it’s best to inject earlier in the day. This will provide a more stable testosterone level throughout the day. However, if you are administering injections less frequently or every two to four weeks, timing may be less critical.

Time of exercise

During exercise, testosterone levels rise. Regular exercise has been associated with higher testosterone levels. For this reason, it’s generally best to inject testosterone a few hours before exercise to maximize its effects. Injecting testosterone after exercise can also be beneficial if your injection schedule allows for it.

Time of day

The time of day you choose for your injection can also play a role in the effectiveness of your treatment. Injecting in the morning can improve sleep quality, give you an energy boost, and make it easier to wake up. However, some people may experience a post-injection energy crash, in which case injecting in the evening may be more suitable.

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Are There Side Effects?

Yes, there are potential side effects to taking Viagra (sildenafil citrate). Some common side effects include:

  • Headache
  • Stomach upset
  • Flushing
  • Dizziness
  • Vision changes, such as increased sensitivity to light and blurred vision

Some men may experience severe side effects. These can include priapism, which is an erection that doesn’t go away, sudden vision loss, or sudden hearing loss.

What Should I Do In Cases Of Emergencies Or Overdose of Testosterone?

If you or a friend have taken too much testosterone and start seeing blurry, having seizures, not being able to see for a little while, feeling suddenly weak, or not being able to talk, it’s super important to get help from a doctor right away.

If a doctor gave you the testosterone, it’s not likely that you took too much. But it’s still important to watch out for side effects and tell your doctor if you feel anything weird or bad.

Using too much testosterone can be really dangerous. It can lead to serious problems like a heart attack, heart failure, feeling really sad (depression), and even death. It’s important to only use testosterone the way your doctor tells you to. Misusing it can be life-threatening.

If it’s an emergency, call the emergency number and follow what they tell you to do. Stay calm and give any important information to the medical team. Remember, acting quickly can save a life.


When you’re thinking about when to take your testosterone shot, there are a few things to consider.

Your age, how quickly you want to see results, and how long the medicine stays in your body can all affect how well the treatment works. Even though you might want to decide on your own, it’s really important to talk to a doctor to figure out the right amount and timing for you.

You might have side effects like acne, more hair growth, changes in your mood, and changes in your sex drive. But your doctor can change your medicine to help with any bad reactions.

Thinking carefully and talking regularly with your doctor can help. Taking testosterone can improve your overall health and how you feel.

Dave Moffat

Hi, I'm Dave Moffat the founder and Chief Editor of and certified International Personal Trainer and Certified Nutritionist. My passion has always been bodybuilding but with 15 years' experience in weight loss programs too, it's hard not to mention all that when you're working at your fitness level fullest (I hope). When Im not in the gym or spending time away from my family i often think about what advice would help others achieve theirs goals just like these inspired mine.